Honours Project July Update

Jack Simpson | July 14th, 2013 | Popular | Research | Varroa

We had a few set-backs with amplifying the Varroa jacobsoni mitochondrial DNA and isolating the fragments which were of the correct size (we needed approximately a 10 kb and 6 kb fragment from a mite from each population in order to get the whole genome). However, once we were able to achieve this and digest the DNA (cut it into smaller sized fragments), the next step involved the transformation of bacteria so they could uptake the DNA fragments we had amplified (and cut up) and make lots of copies of them. The pictures below show the colonies we were recently able to create containing the mite DNA.



Colonies with 6 kb fragments from Varroa jacobsoni on Apis cerana

Colonies with 6 kb fragments from Varroa jacobsoni on Apis cerana


Colonies with 10 kb fragments from Varroa jacobsoni on Apis cerana

Colonies with 10 kb fragments from Varroa jacobsoni on Apis cerana


Colonies with 6 kb fragments from Varroa jacobsoni on Apis mellifera

Colonies with 6 kb fragments from Varroa jacobsoni on Apis mellifera


Colonies with 10 kb fragments from Varroa jacobsoni on Apis mellifera

Colonies with 10 kb fragments from Varroa jacobsoni on Apis mellifera


I’ve scraped out the individual colonies and have started to run some PCRs on the to amplify the inserted mite DNA so hopefully if that goes well I’ll be able to send away some samples to be sequenced very soon.

  • mitelover69

    Looks sexy as hell

  • Thomas J.

    Very good work!!!!

  • Bru and Kat

    Mitey good work
    Love from Mum and Dad xxxxxxx